Angiostrongylus check for this species in other resources - polymerase chain reaction - restriction fragment length polymorphism - second internal transcribed spacer - cytochrome oxidase I"/>
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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 98, No. 8, 2003, pp. 1039-1043
Bioline Code: oc03203
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 98, No. 8, 2003, pp. 1039-1043

 en Molecular Differentiation of Angiostrongylus costaricensis check for this species in other resources , A. cantonensis, and A. vasorum by Polymerase Chain Reaction- Restriction Fragment Length Polymorphism
Roberta L Caldeira; Omar S Carvalho; Cristiane LFG Mendonça; Carlos Graeff-Teixeira; Márcia CF Silva; Renata Ben; Rafael Maurer; Walter S Lima & Henrique L Lenzi

Abstract

Angiostrongylus cantonensis check for this species in other resources , A. costaricensis, and A. vasorum are etiologic agents of human parasitic diseases. Their identification, at present, is only possible by examining the adult worm after a 40-day period following infection of vertebrate hosts with the third-stage larvae. In order to obtain a diagnostic tool to differentiate larvae and adult worm from the three referred species, polymerase chain reaction-restriction fragment length polymorphism was carried out. The rDNA second internal transcribed spacer (ITS2) and mtDNA cytochrome oxidase I regions were amplified, followed by digestion of fragments with the restriction enzymes RsaI, HapII, AluI, HaeIII, DdeI and ClaI. The enzymes RsaI and ClaI exhibited the most discriminating profiles for the differentiation of the regions COI of mtDNA and ITS2 of rDNA respectively. The methodology using such regions proved to be efficient for the specific differentiation of the three species of Angiostrongylus under study.

Keywords
Angiostrongylus check for this species in other resources - polymerase chain reaction - restriction fragment length polymorphism - second internal transcribed spacer - cytochrome oxidase I

 
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