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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 99, No. 1, 2004, pp. 53-55
Bioline Code: oc04009
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 99, No. 1, 2004, pp. 53-55

 en Whole Blood Assay to Access T Cell-immune Responses to Mycobacterium tuberculosis check for this species in other resources Antigens in Healthy Brazilian Individuals
Paulo RZ Antas; Fernando LL Cardoso; Eliane B Oliveira; Patrícia KC Gomes; Kátia S Cunha; Euzenir N Sarno & Elizabeth P Sampaio

Abstract

The production of interferon γ (IFNγ) guarantees effective T cell-mediated immunity against Mycobacterium tuberculosis check for this species in other resources infection. In the present study, we simply compare the in vitro immune responses to Mycobacterium antigens in terms of IFNγ production in a total of 10 healthy Brazilian volunteers. Whole blood and mononuclear cells were cultivated in parallel with PPD, Ag85B, and M. bovis hsp65, and five-days supernatants were harvested for cytokine detection by ELISA. The inter-assay result was that the overall profile of agreement in response to antigens was highly correlated (r2 = 0.9266; p = 0.0102). Potential analysis is in current progress to dictate the usefulness of this method to access the immune responses also in tuberculosis patients and its contacts.

Keywords
tuberculosis (TB) - T cell-responses - whole blood - PPD - latent TB infection - interferon γ

 
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