The production of interferon γ (IFNγ) guarantees effective T cell-mediated immunity against Mycobacterium tuberculosis
infection. In the present study, we simply compare the in vitro immune responses to Mycobacterium antigens
in terms of IFNγ production in a total of 10 healthy Brazilian volunteers. Whole blood and mononuclear cells were cultivated in parallel with PPD, Ag85B, and M. bovis
hsp65, and five-days supernatants were harvested for cytokine detection by ELISA. The inter-assay result was that the overall profile of agreement in response to antigens was highly correlated (r2
= 0.9266; p = 0.0102). Potential analysis is in current progress to dictate the usefulness of this method to access the immune responses also in tuberculosis patients and its contacts.