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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 99, No. 1, 2004, pp. 63-67
Bioline Code: oc04011
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 99, No. 1, 2004, pp. 63-67

 en Plasminogen Interaction with Trypanosoma cruzi check for this species in other resources
Laura Almeida; Gilmer Vanegas; Marina Calcagno; Juan Luis Concepción & Luisana Avilan

Abstract

The ability of Trypanosoma cruzi check for this species in other resources to interact with plasminogen, the zimogenic form of the blood serin protease plasmin, was examined. Immunohistochemistry studies revealed that both forms, epimastigotes and metacyclic trypomastigotes, were able to fix plasminogen in a lysine dependant manner. This interaction was corroborated by plasminogen activation studies. Both forms of the parasite enhanced the plasminogen activation by tissue-type plasminogen activator. The maximal enhancements obtained were 15-fold and 3.4-fold with epimastigotes and metacyclic trypomastigotes, respectively, as compared to plasminogen activation in absence of cells. Ligand-blotting analysis of proteins extracted with Triton X-114 from a microsomal fraction of epimastigotes revealed at least five soluble proteins and one hydrophobic protein able to bind plasminogen.

Keywords
Trypanosoma cruzi check for this species in other resources - plasminogen - plasminogen activation

 
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