Biomphalaria tenagophila check for this species in other resources - polymerase chain reaction and restriction fragment length polymorphism - dominant character - Taim, Rio Grande do Sul - Brazil"/>
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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 99, No. 1, 2004, pp. 85-87
Bioline Code: oc04015
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 99, No. 1, 2004, pp. 85-87

 en Short Communication - Dominant Character of the Molecular Marker of a Biomphalaria tenagophila check for this species in other resources Strain (Mollusca: Planorbidae) Resistant to Schistosoma mansoni check for this species in other resources
Florence Mara Rosa; Roberta Lima Caldeira; Omar dos Santos Carvalho; Ana Lúcia Brunialti Godard & Paulo Marcos Zech Coelho

Abstract

Biomphalaria tenagophila check for this species in other resources population from Taim (state of Rio Grande do Sul, Brazil) is totally resistant to Schistosoma mansoni check for this species in other resources , and presents a molecular marker of 350 bp by polymerase chain reaction and restriction fragment length polymorphism of the entire rDNA internal transcriber spacer. The scope of this work was to determine the heritage pattern of this marker. A series of cross-breedings between B. tenagophila from Taim (resistant) and B. tenagophila from Joinville, state of Santa Catarina (susceptible) was carried out, and their descendants F1 and F2 were submitted to this technique. It was possible to demonstrate that the specific fragment from Taim is endowed with dominant character, since the obtained segregation was typically mendelian.

Keywords
Biomphalaria tenagophila check for this species in other resources - polymerase chain reaction and restriction fragment length polymorphism - dominant character - Taim, Rio Grande do Sul - Brazil

 
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