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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 99, No. 5, 2004, pp. 499-502
Bioline Code: oc04107
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 99, No. 5, 2004, pp. 499-502

 en Diagnostic of Biomphalaria check for this species in other resources Snails and Schistosoma mansoni check for this species in other resources : DNA Obtained from Traces of Shell Organic Materials
Roberta L Caldeira; Liana K Jannotti-Passos; Pollanah M Lira & Omar S Carvalho

Abstract

Freshwater snails belonging to the genus Biomphalaria check for this species in other resources act as intermediate hosts for the parasite trematode Schistosoma mansoni check for this species in other resources in Africa and in the neotropical region. Identification of such molluscs is carried out based on morphological characters and the presence of cercariae is verified through squeezing snails between two glass slides or by exposing them to artificial light. However, sometimes, the material collected includes molluscs with decomposed bodies or, yet, only empty shells, which precludes their identification and S. mansoni detection. Due to these difficulties, we have developed a methodology in which DNA may be extracted from traces of organic material from inside shells in order to identify molluscs through polymerase chain reaction and restriction fragment length polymorphism and to detect S. mansoni into these snails, by using low stringency polymerase chain reaction. Species-specific profiles obtained from B. glabrata, B. straminea, and B. tenagophila snails and their shells, maintained in laboratory for ten years, showed the same profiles. S. mansoni profiles showed to be present in shell specimens as far as the eighth week after being removed from aquarium.

Keywords
Biomphalaria - Schistosoma mansoni - molluscs - shell - DNA - polymerase chain reaction

 
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