en

Detection of hepatitis C virus RNA by in situ hybridization in paraformaldehyde fixed biopsies H. S. Carvalho; M. L. Baptista; M. A. Pinto; M. Silva; C. M. Takiya; V. Chagas; V. Pannain; H. S. M. Coelho & C. F. T. Yoshida Abstract Fourteen hepatitis C virus (HCV) chronically infected patients were submitted to routine liver biopsy for histological evaluation. Liver samples were assayed to HCV-RNA by in situ hybridization, using digoxigenin labeled probe. HCV genotypes were found to be predominantly type 1 (71.4%), followed by genotype 3 (21.4%), and genotype 2 (7.2%). Alanine-aminotransferase levels were raised in 10 patients. The histopathological scores were minimal (21.4%), mild (57.2%), and moderate (21.4%). Viral RNA was detected in liver cells from nine patients (64.3%). ISH method provides localization and poor confirmation of HCV RNA in the liver tissue of HCV chronic patients. Keywords hepatitis C virus RNA - in situ hybridization - liver biopsy - chronic hepatitis - viral hepatitis

© Copyright 2005 Instituto Oswaldo Cruz - Fiocruz
Alternative site location: http://memorias.ioc.fiocruz.br