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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 100, No. 5, 2005, pp. 495-500
Bioline Code: oc05121
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 100, No. 5, 2005, pp. 495-500

 en Identification of Anopheles ( Nyssorhynchus) albitarsis check for this species in other resources complex species (Diptera: Culicidae) using rDNA internal transcribed spacer 2-based polymerase chain reaction primes
Cong Li & Richard C Wilkerson

Abstract

Anopheles ( Nyssorhynchus) marajoara check for this species in other resources is a proven primary vector of malaria parasites in Northeast Brazil, and An. deaneorum is a suspected vector in Western Brazil. Both are members of the morphologically similar Albitarsis Complex, which also includes An. albitarsis and an undescribed species, An. albitarsis "B". These four species were recognized and can be identified using random amplified polymorphic DNA (RAPD) markers, but various other methodologies also point to multiple species under the name An. albitarsis. We describe here a technique for identification of these species employing polymerase chain reaction (PCR) primers based on ribosomal DNA internal transcribed spacer 2 (rDNA ITS2) sequence. Since this method is based on known sequence it is simpler than the sometimes problematical RAPD-PCR. Primers were tested on samples previously identified using RAPD markers with complete correlation.

Keywords
Anopheles albitarsis - species complex - polymerase chain reaction identification - malaria - internal transcribed spacer 2

 
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