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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 100, No. 5, 2005, pp. 555-561
Bioline Code: oc05129
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 100, No. 5, 2005, pp. 555-561

 en Participation of cytokines in the necrotic-inflammatory lesions in the heart and skeletal muscles of Calomys callosus check for this species in other resources infected with Trypanosoma cruzi check for this species in other resources
Isis F Magalhães-Santos & Sonia G Andrade

Abstract

Calomys callosus check for this species in other resources , a sylvatic reservoir of Trypanosoma cruzi check for this species in other resources , when infected with the Colombian strain (Biodeme Type III, T. cruzi I ) develops necrotic-inflammatory lesions and intense early fibrogenesis in the heart and skeletal muscles, that spontaneously regress. Participation of pro-inflammatory and pro-fibrogenic cytokines, such as tumor necrosis factor-alpha (TNF-α , gamma interferon (IFN-γ) , and tumor growth factor-beta (TGF-β), in the pathogenesis of the lesions is herein studied. Eighty C. callosus weighing 20 to 30 g were used. Seventy of them were inoculated with the Colombian strain (105 blood forms) and 10 were maintained as intact non-infected controls. After infection, C. callosus were sacrificed at different time-points from 15 to 70 days. The heart and skeletal muscle were processed for histopathology and cryopreserved for immunohistochemistry. Early necrotic lesions of parasitized skeletal muscle and myocardium with intense inflammatory lesions were present. Search for the in situ presence of TNF-α and IFN-γ, was performed using rat-IgG anti-mouse antibodies against these cytokines. For the in situ search of TGF-β, rabbit IgG anti-mouse antibodies were used. Immunolabeling of the cytokines in tissues of infected C. callosus was successful. The cytokines TNF-α, IFN-γ, and TGF-β were detected in the cytoplasm of macrophages and in the necrotic material from 15 to 45 days post-infection, decreasing their intensity until complete disappearance by the 65th day, which correlated with subsiding histopathological lesions. These findings suggest the participation of these cytokines in the control of parasite multiplication, in the development of an early fibrogenesis and in the regression of fibrotic-inflammatory lesions observed in C. callosus.

Keywords
Trypanosoma cruzi - Calomys callosus - cytokines - tumor necrosis factor-alpha - gamma interferon - tumor growth factor-beta

 
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