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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 100, No. 7, 2005, pp. 749-752
Bioline Code: oc05158
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 100, No. 7, 2005, pp. 749-752

 en SHORT COMMUNICATION- Mycobacterium bovis check for this species in other resources identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil
Cristina Pires de Araújo; Clarice Queico Fugimura Leite; Karina Andrade de Prince; Klaudia dos Santos Gonçalves Jorge & Ana Luiza Alves Rosa Osório

Abstract

The presence of Mycobacterium bovis check for this species in other resources in bovine carcasses with lesions suggestive of tuberculosis was evaluated. Seventy-two carcass samples were selected during slaughter inspection procedures in abattoirs in the state of Mato Grosso do Sul, Brazil. Seventeen (23.6%) of samples showed colonies suggestive of mycobacteria that were confirmed to be acid-fast bacilli by Ziehl-Neelsen staining. Polymerase chain reaction (PCR) using primers specific for M. bovis identified M. bovis in 13 (76.5%) isolates. The PCR-restriction enzyme pattern analysis using gene encoding for the 65-kDa protein and two restriction enzymes identified the remaining four isolates that were represented by two M. tuberculosis complex and two nontuberculous mycobacteria. The results are indicative of infection of slaughter cattle by M. bovis and other mycobacteria in the state of Mato Grosso do Sul.

Keywords
Mycobacterium bovis - nontuberculous mycobacteria - polymerase chain reaction - PCR-restriction enzyme pattern analysis - cattle - post-mortem inspection - abattoirs

 
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