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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 100, No. 8, 2005, pp. 875-881
Bioline Code: oc05177
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 100, No. 8, 2005, pp. 875-881

 en Clinical and pathological importance of vacA allele heterogeneity and cagA status in peptic ulcer disease in patients from North Brazil
Luisa Caricio Martins; Tereza Cristina de Oliveira Corvelo; Samia Demachki; Marialva TF Araujo; Mônica Baraúna Assumpção; Simone Cristina Araujo Jucá Vilar; Felipe Bonfim Freitas; Hivana Patricia Melo Barbosa; Amanda Alves Fecury; Renata Kelly Costa do Amaral & Sidney Emanuel Batista dos Santos

Abstract

We have examined the prevalence of gene cagA and vacA alleles in 129 patients, 69 with gastritis and 60 with peptic ulcer diseases from North Brazil and their relation with histopathological data. vacA and cagA genotype were determined by polymerase chain reaction. Hematoxylin-eosin staining was used for histological diagnosis. 96.6% of the patients were colonized by Helicobacter pylori check for this species in other resources strains harboring single vacA genotype (nont-mixed infection). Among them, 11.8% had subtype s1a, 67.8% had subtype s1b, and 17% subtype s2. In regard to the middle region analysis, m1 alleles were found in 75.4% and m2 in 21.2% of patients. The cagA gene was detected in 78% patients infected with H. pylori and was associated with the s1-m1 vacA genotype. The H. pylori strains, vacA s1b m1/cagA-positive, were associated with increased risk of peptic ulcer disease and higher amounts of lymphocytic and neutrophilic infiltrates and the presence of intestinal metaplasia. These findings show that cagA and vacA genotyping may have clinical relevance in Brazil.

Keywords
Helicobacter pylori - peptic ulcer - vacA alleles - cagA status - Brazil

 
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