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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 101, No. 3, 2006, pp. 339-340
Bioline Code: oc06061
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 101, No. 3, 2006, pp. 339-340

 en A micro-spreading improvement for spermatogenic chromosomes from Triatominae (Hemiptera-Reduviidae)
Mauricio Camargo; María Adelaida Duque-Correa & Alejandro Berrío

Abstract

Cytogenetics of triatomines have been a valuable biological tool for the study of evolution, taxonomy, and epidemiology of these vectors of Trypanosoma cruzi check for this species in other resources . Here we present a single microtube protocol that combines micro-centrifugation and micro-spreading, allowing high quality cytogenetic preparations from male gonadal material of Rhodnius prolixus check for this species in other resources and Triatoma lecticularia check for this species in other resources . The amount of cellular scattering can be modulated, which can be useful if small aggregates of synchronous cells are desired. Moreover, a higher number of slides per gonad can be obtained with fully flattened clean chromosomal spreads with minimum overlaps, optimal for classical and modern molecular cytogenetic analyses.

Keywords
micro-spreading - Triatominae - chromosomes

 
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