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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 101, No. 5, 2006, pp. 511-516
Bioline Code: oc06086
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 101, No. 5, 2006, pp. 511-516

 en Comparison between indirect enzyme-linked immunosorbent assays for Anaplasma marginale check for this species in other resources antibodies with recombinant major surface protein 5 and initial body antigens
Virgínia MG Silva; Flábio R Araújo; Claudio R Madruga; Cleber O Soares; Raul H Kessler; Maria AO Almeida; Stênio P Fragoso; Lenita R Santos; Carlos AN Ramos; Gisele Bacanelli & Roberto AA Torres Júnior

Abstract

Indirect enzyme-linked immunosorbent assays (ELISAs) based on recombinant major surface protein 5 (rMSP5) and initial body (IB) antigens from a Brazilian isolate of Anaplasma marginale check for this species in other resources were developed to detect antibodies against this rickettsia in cattle. Both tests showed the same sensitivity (98.2%) and specificities (100% for rMSP5 and 93.8% for IB ELISA) which did not differ statistically. No cross-reactions were detected with Babesia bigemina check for this species in other resources antibodies, but 5 (rMSP5 ELISA) to 15% (IB ELISA) of cross-reactions were detected with B. bovis antibodies. However, such difference was not statistically significant. Prevalences of seropositive crossbred beef cattle raised extensively in Miranda county, state of Mato Grosso do Sul, Brazil, were 78.1% by rMSP5 ELISA and 79.7% by IB ELISA. In the analysis of sera from dairy calves naturally-infected with A. marginale, the dynamics of antibody production was very similar between both tests, with maternal antibodies reaching the lowest levels at 15-30 days, followed by an increase in the mean optical densities in both ELISAs, suggesting the development of active immunity against A. marginale. Results showed that all calves were seropositive by one-year old, characterizing a situation of enzootic stability. The similar performances of the ELISAs suggest that both tests can be used in epidemiological surveys for detection of antibodies to A. marginale in cattle.

Keywords
Anaplasma marginale - enzyme-linked immunosorbent assay - major surface protein 5 - initial body - Brazil

 
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