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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 101, No. 6, 2006, pp. 639-643
Bioline Code: oc06108
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 101, No. 6, 2006, pp. 639-643

 en Primary culture of the region of the amebocyte-producing organ of the snail Biomphalaria glabrata check for this species in other resources , the intermediate host of Schistosoma mansoni check for this species in other resources
L Barbosa; LM Silva; PMZ Coelho; SR Santos & CL Fortes-Dias

Abstract

Biomphalaria glabrata check for this species in other resources snails are major hosts for the digenetic trematoda Schistosoma mansoni check for this species in other resources , the causative agent of human schistosomiasis. The success or failure of the infection will be dependent on the mobilization of the molluskan internal defense system, where a major role will be played by circulating hemocytes produced by the APO (amebocyte-producing organ) of the snail. In this report, the primary culture of the APO region of B. glabrata was obtained for the first time, as well as a control culture of the ovotestis. Three different cell populations migrated easily from the explants in culture, with no need of any dispersion agent. The cells grew in suspension at an incubation temperature of 15°C and the cultures were maintained viable for up to two weeks. Two of these cell populations obtained resembled cell types known to be present in the hemolymph of Biomphalaria. The availability of APO cells in culture may contribute to a better understanding of the internal defense in mollusks, in general, as well as the specific response of B. glabrata to S. mansoni infection.

Keywords
Biomphalaria - schistosomiasis - Schistosoma mansoni - hemocyte - tissue culture

 
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