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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 101, No. 7, 2006, pp. 755-757
Bioline Code: oc06126
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 101, No. 7, 2006, pp. 755-757

 en Germline transformation of Aedes fluviatilis check for this species in other resources (Diptera:Culicidae) with the piggyBac transposable element
Flávia Guimarães Rodrigues; Sabrina Barbosa Oliveira; Bruno Coelho Rocha & Luciano Andrade Moreira

Abstract

The technique to generate transgenic mosquitoes requires adaptation for each target species because of aspects related to species biology, sensitivity to manipulation and rearing conditions. Here we tested different parameters on the microinjection procedure in order to obtain a transgenic Neotropical mosquito species. By using a transposon-based strategy we were able to successfully transform Aedes fluviatilis check for this species in other resources (Lutz), which can be used as an avian malaria model. These results demonstrate the usefulness of the piggyBac transposable element as a transformation vector for Neotropical mosquito species and opens up new research frontiers for South American mosquito vectors.

Keywords
transgenic mosquitoes - piggyBac - malaria

 
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