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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 101, No. s1, 2006, pp. 365-368
Bioline Code: oc06218
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 101, No. s1, 2006, pp. 365-368

 en Aluminum hydroxide associated to Schistosoma mansoni check for this species in other resources 22.6 kDa protein abrogates partial protection against experimental infection but not alter interleukin-10 production
Lucila GG Pacífico; Cristina T Fonseca; Michele M Barsante; Luciana S Cardoso; Maria Ilma Araújo & Sérgio C Oliveira

Abstract

The need to develop a vaccine against schistosomiasis led several researches and our group to investigate proteins from Schistosoma mansoni check for this species in other resources as vaccine candidates. Sm22.6 is a protein from S. mansoni that shows high identity with Sj22.6 and Sh22.6 (79 and 91%, respectively). These proteins are associated with high levels of IgE and protection to reinfection. Previously, we have shown that Sm22.6 induced a partial protection of 34.5% when used together with Freund's adjuvant and produced a Th0 type of immune response with interferon-g and interleukin-4. In this work, mice were immunized with Sm22.6 alone or with aluminum hydroxide adjuvant and high levels of IgG, IgG1, and IgG2a were measured. Unfortunately, no protection was detected. Since IL-10 is a modulating cytokine in schistosomiasis, we also observed a high level of this molecule in splenocytes of vaccinated mice. In conclusion, we did not observe the adjuvant effect of aluminum hydroxide associated with rSm22.6 in protective immunity.

Keywords
Schistosoma mansoni - Sm22.6 - recombinant vaccine - aluminum hydroxide - adjuvant - interleukin-10

 
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