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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 102, No. 4, 2007, pp. 497-500
Bioline Code: oc07078
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 102, No. 4, 2007, pp. 497-500

 en Sensitivity and specificity of polymerase chain reaction in Giemsastained slides for diagnosis of visceral leishmaniasis in children
Brustoloni, Yvone Maia; Lima, Rosimar Batista; da Cunha, Rivaldo Venâncio; Dorval, Maria Elizabeth; Oshiro, Elisa Teruya; de Oliveira, Ana Lúcia Lyrio & Pirmez, Claude

Abstract

The aim of this study was to evaluate the sensitivity and specificity of polymerase chain reaction (PCR) in the detection of Leishmania DNA in archived Giemsa-stained bone marrow slides for diagnosis of visceral leishmaniasis (VL), and to compare PCR with conventional diagnostic techniques, like direct microscopy and parasite culture. Specimens of archived Giemsa-stained bone marrow slides from 91 patients with VL and from 79 controls with other diseases or conditions were studied. PCR showed the highest sensitivity (92.3%) and had good specificity (97.5%). Direct examination detected 79.1% and culture 59% of positive samples. In addition, PCR was able to detect VL in 16 of 19 patients (84.2%) with negative microscopy. PCR in Giemsa-stained bone marrow slides is a suitable tool for confirming diagnosis in patients with VL and may be useful in the diagnosis of difficult cases. Slide smears are easily stored, do not require special storage conditions such as low temperatures, and can be easily mailed to centers where PCR is available, making it an excellent option for diagnosis in the field.

Keywords
visceral leishmaniasis - polymerase chain reaction - diagnosis

 
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