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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 102, No. 4, 2007, pp. 509-515
Bioline Code: oc07080
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 102, No. 4, 2007, pp. 509-515

 en Cloning and characterization of a V-ATPase subunit C from the American visceral leishmaniasis vector Lutzomyia longipalpis check for this species in other resources modulated during development and blood ingestion
Ramalho-Ortigão, JM; Pitaluga, AN; Telleria, EL; Marques, C; Souza, AA & Traub-Cseko, YM


Visceral leishmaniasis (VL) is a serious tropical disease that affects approximately 500 thousand people worldwide every year. In the Americas, VL is caused by the parasite Leishmania (Leishmania) infantum chagasi check for this species in other resources mainly transmitted by the bite of the sand fly vector Lutzomyia longipalpis. Despite recent advances in the study of interaction between Leishmania and sand flies, very little is known about sand fly protein expression profiles. Understanding how the expression of proteins may be affected by blood feeding and/or presence of parasite in the vector's midgut might allow us to devise new strategies for controlling the spread of leishmaniasis. In this work, we report the characterization of a vacuolar ATPase subunit C from L. longipalpis check for this species in other resources by screening of a midgut cDNA library with a 220 bp fragment identified by means of differential display reverse transcriptasepolymerase chain reaction analysis. The expression of the gene varies along insect development and is upregulated in males and bloodfed L. longipalpis, compared to unfed flies.

Lutzomyia longipalpis - V-ATPase - metacyclogenesis - differential expression

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