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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 103, No. 1, 2008, pp. 31-38
Bioline Code: oc08005
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 103, No. 1, 2008, pp. 31-38

 en Screening of Amazonian plants from the Adolpho Ducke forest reserve, Manaus, state of Amazonas, Brazil, for antimicrobial activity
Carneiro, Ana Lúcia Basílio; Teixeira, Maria Francisca Simas; de Oliveira, Viviana Maria Araújo; Fernandes, Ormezinda Celeste Cristo; Cauper, Gláucia Socorro de Barros & Pohlit, Adrian Martin

Abstract

Tropical forests are species-rich reserves for the discovery and development of antimicrobial drugs. The aim of this work is to investigate the in vitro antimicrobial potential of Amazon plants found within the National Institute on Amazon Research's Adolpho Ducke forest reserve, located in Manaus, state of Amazonas, Brazil. 75 methanol, chloroform and water extracts representing 12 plant species were tested for antimicrobial activity towards strains of Mycobacterium smegmatis check for this species in other resources , Escherichia coli check for this species in other resources , Streptococcus sanguis check for this species in other resources , Streptococcus oralis check for this species in other resources , Staphylococcus aureus check for this species in other resources and Candida albicans check for this species in other resources using the gel-diffusion method. Active extracts were further evaluated to establish minimum inhibitory concentrations (MIC) and antimicrobial profiles using bioautography on normal-phase thin-layer chromatography plates. Diclinanona calycina check for this species in other resources presented extracts with good antimicrobial activity and S. oralis and M. smegmatis were the most sensitive bacteria. D. calycina and Lacmellea gracilis check for this species in other resources presented extracts with the lowest MIC (48.8 μg/ml). D. calycina methanol and chloroform leaf extracts presented the best overall antimicrobial activity. All test organisms were sensitive to D. calycina branch chloroform extract in the bioautography assay. This is the first evaluation of the biological activity of these plant species and significant in vitro antimicrobial activity was detected in extracts and components from two species, D. calycina and L. gracilis.

Keywords
plant extract - antibacterial - antifungal - Diclinanona calycina - Lacmellea gracilis - bioautography

 
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