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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 103, No. 2, 2008, pp. 172-179
Bioline Code: oc08029
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 103, No. 2, 2008, pp. 172-179

 en Preliminary characterization of a Rhodnius prolixus check for this species in other resources hemolymph trypanolytic protein, this being a determinant of Trypanosoma rangeli check for this species in other resources KP1(+) and KP1(-) subpopulations' vectorial ability
Pulido, Ximena Carolina; Pérez, Gerardo & Vallejo, Gustavo Adolfo

Abstract

Rhodnius prolixus check for this species in other resources is the main Trypanosoma rangeli check for this species in other resources vector in several Latin-American countries and is susceptible to infection with KP1(+) strains; however, it presents an invasion-resistant response to KP1(-) strains. The present work has identified a trypanolytic protein against T. rangeli KP1(-) in the R. prolixus hemolymph which was fractioned with ammonium sulfate (following dialysis). The results revealed a protein component which did not depend on divalent cations for its biological function whilst keeping its trypanolytic activity at temperatures ranging from -20ºC to 37ºC, at 7.0 to 10.5 pH. The protein was partially purified by gel filtration chromatography and ionic exchange chromatography. The major component presented a molecular weight of around 79 kDa and an isoelectric point between 4.9 and 6.3 and may be directly related to hemolymph trypanolytic activity against T. rangeli KP1(-) populations.

Keywords
Rhodnius prolixus - Trypanosoma rangeli KP1(+) - Trypanosoma rangeli KP1(-) - trypanolytic factor - vectorial ability - insect immunity

 
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