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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 103, No. 5, 2008, pp. 455-462
Bioline Code: oc08080
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 103, No. 5, 2008, pp. 455-462

 en Regional pattern of the molecular types of Cryptococcus neoformans check for this species in other resources and Cryptococcus gattii check for this species in other resources in Brazil
Trilles, Luciana; Lazéra, Márcia dos Santos; Wanke, Bodo; Oliveira, Raquel Vasconcelos; Barbosa, Gláucia Gonçalves; Nishikawa, Marília Martins; Morales, Bernardina Penarrieta & Meyer, Wieland

Abstract

The molecular types of 443 Brazilian isolates of Cryptococcus neoformans check for this species in other resources and Cryptococcus gattii check for this species in other resources were analyzed to determine their geographic distribution within Brazil and their underlying host conditions. The following data, imported from previous epidemiological studies as well as two culture collections, were analyzed for: place of isolation, source (clinical or environmental), host risk factors, species, serotype, mating type, and molecular type. Molecular typing by PCR-fingerprinting using primers for the minisatellite-specific core sequence of the wild-type phage M13 or microsatellites [(GACA)4, (GTG)5], restriction fragment length polymorphism of URA5 gene analysis, and/or amplified fragment length polymorphism (AFLP) identified eight major genotypes: VNI/AFLP1, VNII/AFLP1A, VNIII/AFLP2, and VNIV/AFLP3 for C. neoformans, and VGI/AFLP4, VGII/AFLP6, VGIII/AFLP5, and VGIV/AFLP7 for C. gattii. The most common molecular type found in Brazil was VNI (64%), followed by VGII (21%), VNII (5%), VGIII (4%), VGI and VNIV (3% each), and VNIII (< 1%). Primary cryptococcosis caused by the molecular type VGII (serotype B, MAT) prevails in immunocompetent hosts in the North and Northeast regions, disclosing an endemic regional pattern for this specific molecular type in the Northern Brazil.

Keywords
Cryptococcus neoformans - Cryptococcus gattii - molecular types - epidemiology - Brazil

 
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