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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 104, No. 5, 2009, pp. 710-714
Bioline Code: oc09159
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 104, No. 5, 2009, pp. 710-714

 en In house colorimetric reverse hybridisation assay for detection of the mutation most frequently associated with resistance to isoniazid in Mycobacterium tuberculosis check for this species in other resources
Verza, Mirela; Maschmann, Raquel de Abreu; Silva, Márcia Susana Nunes; Costa, Elis Regina Dalla; Ribeiro, Marta Osório; Rosso, Franciele; Suffys, Philip Noel; Tortoli, Enrico; Marcelli, Fiorella; Zaha, Arnaldo & Rossetti, Maria Lucia Rosa

Abstract

Mutations in the katG gene have been identified and correlated with isoniazid (INH) resistance in Mycobacterium tuberculosis check for this species in other resources isolates. The mutation AGC→ACC (Ser→Thr) at katG315 has been reported to be the most frequent and is associated with transmission and multidrug resistance. Rapid detection of this mutation could therefore improve the choice of an adequate anti-tuberculosis regimen, the epidemiological monitoring of INH resistance and, possibly, the tracking of transmission of resistant strains. An in house reverse hybridisation assay was designed in our laboratory and evaluated with 180 isolates of M. tuberculosis. It could successfully characterise the katG315 mutation in 100% of the samples as compared to DNA sequencing. The test is efficient and is a promising alternative for the rapid identification of INH resistance in regions with a high prevalence of katG315 mutants.

Keywords
drug resistance - isoniazid - katG315 - reverse hybridization assay - tuberculosis

 
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