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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 104, No. 8, 2009, pp. 1194-1196
Bioline Code: oc09239
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 104, No. 8, 2009, pp. 1194-1196

 en Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni check for this species in other resources DNA in human stool samples
Gomes, Luciana I; Marques, Letícia HS; Enk, Martin J; Coelho, Paulo Marcos Z & Rabello, Ana

Abstract

A previously reported sensitive PCR assay for the detection of Schistosoma mansoni check for this species in other resources DNA was updated and evaluated. Changes in the DNA extraction method, including the use of a worldwide available commercial kit and the inclusion of additional quality control measures, increased the robustness of the test, as confirmed by the analysis of 67 faecal samples from an endemic area in Brazil. The PCR assay is at hand as a proven, reliable diagnostic test for the control of schistosomiasis in specific settings.

Keywords
schistosomiasis - molecular diagnosis - faecal samples - polymerase chain reaction

 
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