Variation among natural populations of
Culex (Culex)
quinquefasciatus
Say is associated with different vectorial capacities. The species
Cx. quinquefasciatus is present in the equatorial, tropical and subtropical zones in the Brazilian territory, with intermediate forms between
Cx. quinquefasciatus and
Culex pipiens
occurring in regions of latitudes around 33°-35°S. Herein, we studied geographically distinct populations of
Cx. quinquefasciatus by genetic characterization and analysis of intra-specific wing morphometrics. After morphological analysis, molecular characterization of
Cx. quinquefasciatus and intermediate forms was performed by polymerase chain reaction of the polymorphic nuclear region of the second intron of the acetylcholinesterase locus. Additionally, the morphology of adult female wings collected from six locations was analyzed. Wing centroid sizes were significantly different between some geographical pairs. Mean values of R
2/R
2+3 differed significantly after pairwise comparisons. The overall wing shape represented by morphometric characters could be divided into two main groupings. Our data suggest that Brazilian samples are morphologically and genetically distinct from the Argentinean samples and also indicated a morphological distinction between northern and southern populations of Brazilian
Cx. quinquefasciatus. We suggest that wing morphology may be used for preliminary assessment of population structure of
Cx. quinquefasciatusin Brazil.
