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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 105, No. 5, 2010, pp. 722-728
Bioline Code: oc10123
Full paper language: English
Document type: Note
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 105, No. 5, 2010, pp. 722-728

 en Evaluation of Lionex TB kits and mycobacterial antigens for IgG and IgA detection in cerebrospinal fluid from tuberculosis meningitis patients
Sardella, Isabela Gama; Singh, Mahavir; Kumpfer, Susanne; Heringer, Rafael Ribeiro; Saad, Maria Helena Féres & Sohler, Marzia Puccioni


To evaluate commercial Lionex TB together with four antigens of Mycobacterium tuberculosis check for this species in other resources (MPT-64, MT10.3, 16 kDa and 38 kDa) for IgG and IgA cerebrospinal fluid (CSF) detection in the diagnosis of tuberculosis meningitis (TBM) with CSF negative acid-fast bacilli staining, 19 cases of TBM, 64 cases of other infectious meningoencephalitis and 73 cases of other neurological disorders were tested by enzyme linked immunosorbent assay. IgA-MPT-64 and IgG Lionex showed the highest sensitivities, specificities, positive predictive value and negative predictive value (63.2%, 47.4%; 95%, 93.7%; 40%, 98% and 28.4%, 97.1%, respectively). However, while grey zone was 12.7% and 6%, respectively, lowering sensitivity but maintains high specificity (≥ 95%). High protein concentration in CSF was associated with antibody positivity CSF/HIV+ which did not influence the sensitivity of both tests. To our knowledge, this is the first description of IgA-MPT-64 and IgG Lionex antibodies in CSF-TBM and, although there is good specificity, adjustments are needed based on antigen composition to enhance sensitivity.

tuberculosis - cerebrospinal fluid - ELISA - meningoencephalitis tuberculosis - Mycobacterium tuberculosis - immune response

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