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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 105, No. 6, 2010, pp. 823-828
Bioline Code: oc10138
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 105, No. 6, 2010, pp. 823-828

 en Antigenic extracts of Leishmania braziliensis check for this species in other resources and Leishmania amazonensis check for this species in other resources associated with saponin partially protects BALB/c mice against Leishmania chagasi check for this species in other resources infection by suppressing IL-10 and IL-4 production
Grenfell, Rafaella F. Q.; Marques-da-Silva, Eduardo A.; Souza-Testasicca, Miriam C.; Coelho, Eduardo A. F.; Fernandes, Ana Paula; Afonso, Luís Carlos C. & Rezende, Simone A.

Abstract

This study evaluated two vaccine candidates for their effectiveness in protecting BALB/c mice against Leishmania chagasi check for this species in other resources infection. These immunogenic preparations were composed of Leishmania amazonensis check for this species in other resources or Leishmania braziliensis check for this species in other resources antigenic extracts in association with saponin adjuvant. Mice were given three subcutaneous doses of one of these vaccine candidates weekly for three weeks and four weeks later challenged with promastigotes of L. chagasi by intravenous injection. We observed that both vaccine candidates induced a significant reduction in the parasite load of the liver, while the L. amazonensis antigenic extract also stimulated a reduction in spleen parasite load. This protection was associated with a suppression of both interleukin (IL)-10 and IL-4 cytokines by spleen cells in response to L. chagasiantigen. No change was detected in the production of IFN-γ. Our data show that these immunogenic preparations reduce the type 2 immune response leading to the control of parasite replication.

Keywords
vaccine - Leishmania chagasi - Leishmania braziliensis - Leishmania amazonensis - IL-10 - IL-4

 
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