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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 106, No. s1, 2011, pp. 34-43
Bioline Code: oc11138
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 106, No. s1, 2011, pp. 34-43

 en Antibodies against the Plasmodium falciparum check for this species in other resources glutamate-rich protein from naturally exposed individuals living in a Brazilian malaria-endemic area can inhibit in vitro parasite growth
Pratt-Riccio, Lilian Rose; Bianco-Junior, Cesare; Totino, Paulo Renato Rivas; Perce-Da-Silva, Daiana De Souza; Silva, Luciene Aquino; Riccio, Evelyn Kety Pratt; Ennes-Vidal, Vítor; Neves-Ferreira, Ana Gisele Costa; Perales, Jonas; Da Rocha, Surza Lucia Gonçalves; Dias-Da-Silva, Fabrício; Ferreira-da-Cruz, Maria de Fátima; Daniel-Ribeiro, Cláudio Tadeu; De Oliveira-Ferreira, Joseli; Theisen, Michael; De Moura Carvalho, Leonardo José & Banic, Dalma Maria


The glutamate-rich protein (GLURP) is an exoantigen expressed in all stages of the Plasmodium falciparum check for this species in other resources life cycle in humans. Anti-GLURP antibodies can inhibit parasite growth in the presence of monocytes via antibody-dependent cellular inhibition (ADCI), and a major parasite-inhibitory region has been found in the N-terminal R0 region of the protein. Herein, we describe the antiplasmodial activity of anti-GLURP antibodies present in the sera from individuals naturally exposed to malaria in a Brazilian malaria-endemic area. The anti-R0 antibodies showed a potent inhibitory effect on the growth of P. falciparum in vitro, both in the presence (ADCI) and absence (GI) of monocytes. The inhibitory effect on parasite growth was comparable to the effect of IgGs purified from pooled sera from hyperimmune African individuals. Interestingly, in the ADCI test, higher levels of tumour necrosis factor alpha (TNF-a) were observed in the supernatant from cultures with higher parasitemias. Our data suggest that the antibody response induced by GLURP-R0 in naturally exposed individuals may have an important role in controlling parasitemia because these antibodies are able to inhibit the in vitro growth of P. falciparum with or without the cooperation from monocytes. Our results also indicate that TNF-a may not be relevant for the inhibitory effect on P. falciparum in vitro growth.

malaria, Plasmodium falciparum, GLURP, antibodies, biological activity, growth inhibition

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