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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 107, No. 1, 2012, pp. 64-73
Bioline Code: oc12009
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 107, No. 1, 2012, pp. 64-73

 en Spoligotyping and variable number tandem repeat analysis of Mycobacterium bovis check for this species in other resources isolates from cattle in Brazil
Parreiras, Patrícia Martins; Andrade, Giovanna Ivo; do Nascimento, Telma de Figueiredo; Oelemann, Maraníbia Cardoso; Gomes, Harrison Magdinier; de Alencar, Andrea Padilha; de Assis, Ronnie Antunes; Mota, Pedro Moacyr Pinto Coelho; Pereira, Márcia Aparecida da Silva; Lobato, Francisco Carlos Faria; Lage, Andrey Pereira & Suffys, Philip Noel

Abstract

We performed spoligotyping and 12-mycobacterial interspersed repetitive unit-variable number tandem repeats(MIRU-VNTRs) typing to characterise Mycobacterium bovis check for this species in other resources isolates collected from tissue samples of bovines with lesions suggestive for tuberculosis during slaughter inspection procedures in abattoirs in Brazil. High-quality genotypes were obtained with both procedures for 61 isolates that were obtained from 185 bovine tissue samples and all of these isolates were identified as M. bovis by conventional identification procedures. On the basis of the spoligotyping, 53 isolates were grouped into nine clusters and the remaining eight isolates were unique types, resulting in 17 spoligotypes. The majority of the Brazilian M. bovis isolates displayed spoligotype patterns that have been previously observed in strains isolated from cattle in other countries. MIRU-VNTR typing produced 16 distinct genotypes, with 53 isolates forming eight of the groups, and individual isolates with unique VNTR profiles forming the remaining eight groups. The allelic diversity of each VNTR locus was calculated and only two of the 12-MIRU-VNTR loci presented scores with either a moderate (0.4, MIRU16) or high (0.6, MIRU26) discriminatory index (h). Both typing methods produced similar discriminatory indexes (spoligotyping h = 0.85; MIRU-VNTR h = 0.86) and the combination of the two methods increased the h value to 0.94, resulting in 29 distinct patterns. These results confirm that spoligotyping and VNTR analysis are valuable tools for studying the molecular epidemiology of M. bovis infections in Brazil.

Keywords
Mycobacterium bovis - spoligotyping - MIRU-VNTR typing - bovine tuberculosis - molecular epidemiology

 
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