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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 108, No. 2, 2013, pp. 172-177
Bioline Code: oc13032
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 108, No. 2, 2013, pp. 172-177

 en Nitric oxide production by Peromyscus yucatanicus check for this species in other resources (Rodentia) infected with Leishmania (Leishmania) mexicana check for this species in other resources
Loría-Cervera, Elsy Nalleli; Sosa-Bibiano, Erika Ivett; Villanueva-Lizama, Liliana Estefanía; Raymonde Van Wynsberghe, Nicole; Canto-Lara, Silvia Beatriz; Batún-Cutz, José Luis & Andrade-Narváez, Fernando José


Peromyscus yucatanicus check for this species in other resources (Rodentia: Cricetidae) is a primary reservoir of Leishmania (Leishmania) mexicana (Kinetoplastida: Trypanosomatidae). Nitric oxide (NO) generally plays a crucial role in the containment and elimination of Leishmania. The aim of this study was to determine the amount of NO produced by P. yucatanicus infected with L. (L.) mexicana. Subclinical and clinical infections were established in P. yucatanicus through inoculation with 1 x 102 and 2.5 x 106 promastigotes, respectively. Peritoneal macrophages were cultured alone or co-cultured with lymphocytes with or without soluble Leishmania antigen. The level of NO production was determined using the Griess reaction. The amount of NO produced was significantly higher (p ≤ 0.0001) in co-cultured macrophages and lymphocytes than in macrophages cultured alone. No differences in NO production were found between P. yucatanicus with subclinical L. (L.) mexicana infections and animals with clinical infections. These results support the hypothesis that the immunological mechanisms of NO production in P. yucatanicus are similar to those described in mouse models of leishmaniasis and, despite NO production, P. yucatanicus is unable to clear the parasite infection.

nitric oxide; Peromyscus yucatanicus; Leishmania (L.) mexicana

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