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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 90, No. 2, 1995, pp. 179-184
Bioline Code: oc95037
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 90, No. 2, 1995, pp. 179-184

 en Molecular Aspects of Schistosoma mansoni check for this species in other resources Female Maturation
Ana Lucia Moraes Giannini; Sergio Vasconcelos Linhares; Elena Cristina Caride; Vania Maria Martins Braga & Franklin David Rumjanek

Abstract

Incubation of total protein extracts of  Schistosoma  mansoni
  check for this species in other resources

with ^3H 17-b-estradiol and 20-hydroxyecdysone, revealed
steroid binding proteins in both, male and female worms. The
interaction of nuclear proteins  with restriction fragments of
the gender and stage-specific gene F-10 was investigated using
the "Band-Shift" technique. Distinct male and female nuclear
proteins bound to the fragments of this gene. Among the
nuclear proteins, only those rich in cysteine residues bound
to DNA.  In vitro incubation of live worms with the estrogen
antagonist Tamoxifen,  altered the pattern of the DNA binding
proteins, producing in females, a band profile similar to that
obtained with male worm protein extracts.  When Tamoxifen was
injected into schistosome infected mice, the eggs produced by
females presented an abnormal morphology, compatible with non-
viable eggs. These results suggest that the regulation of
transcription of the F-10 gene  might involve steroid
receptors.

Keywords
Schistosoma mansoni - nuclear proteins - F-10 gene - steroid receptors - Tamoxifen - eggs

 
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