search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 90, No. 2, 1995, pp. 281-284
Bioline Code: oc95055
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 90, No. 2, 1995, pp. 281-284

 en A Molecular Genetic Study of the Variations in Metabolic Function During Schistosome Development
Patrick J Skelly & Charles B Shoemaker

Abstract

During their complex life cycle schistosomes alternate
between the use of stored glycogen and reliance on host 
glucose to provide for their energy needs. In addition, 
there is dramatic variation between the relative
contribution of aerobic versus anaerobic glucose metabolism 
during development.  We have cloned a set of
representative cDNAs that encode proteins involved in
glucose uptake, glycolysis, Kreb's cycle and oxidative 
phosphorylation.  The different cDNAs were used as
probes to examine the expression of glucose metabolism genes
during the schistosome life cycle. Steady state mRNA levels 
from whole cercariae, isolated cercarial tails, schistosomula 
and adult worms were analysed on Northern blots and dot blots 
which were quantified using storage phosphor technology.  These
studies reveal:  (1)  Transcripts encoding glycogen metabolic 
enzymes are expressed to much higher levels in cercarial tails 
than whole cercariae or schistosomula while the opposite pattern 
is found for glucose transporters and hexokinase transcripts; 
(2)  Schistosomula contain low levels of transcripts
encoding respiratory enzymes but regain the capacity for aerobic 
glucose metabolism as they mature to adulthood;  (3)  Male and 
female adults contain similar levels of the different
transcripts involved in glucose metabolism.

Keywords
schistosome - metabolism - transcription - glucose

 
© Copyright 1995 Fundacao Oswaldo Cruz (Fiocruz)
Alternative site location: http://memorias.ioc.fiocruz.br

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2017, Site last up-dated on 05-Dec-2017.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil