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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 90, No. 6, 1995, pp. 721-732
Bioline Code: oc95147
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 90, No. 6, 1995, pp. 721-732

 en Identification of Species Related to Anopheles (Nyssorhynchus) albitarsis by Random Amplified Polymorphic DNA-Polymerase Chain Reaction (Diptera: Culicidae)
Richard C Wilkerson; Thomas V Gaffigan & Joso Bento Lima


Species-specific Random Amplified Polymorphic DNA-Polymerase
Chain Reaction (RAPD-PCR) markers were used to identify four
species related to Anopheles (Nyssorhynchus) albitarsis
Lynch-Arribalphalzaga from 12 sites in Brazil and 4 in
Venezuela.  In a previous study (Wilkerson et al. 1995), which
included sites in Paraguay and Argentina, these four species
were designated "A", "B", "C" and "D".  It was hypothesized
that species A is An. (Nys.) albitarsis, species B is
undescribed, species C is An. (Nys.) marajoara Galvpio and
Damasceno and species D is An. (Nys.) deaneorum Rosa-Freitas. 
Species D, previously characterized by RAPD-PCR from a small
sample from northern Argentina and southern Brazil, is
reported here from the type locality of An. (Nys.) deaneorum,
Guajarbeta-Mirim,  State of Rondnia, Brazil.  Species C and D
were found by RAPD-PCR to be sympatric at Costa Marques, State
of Rondonia, Brazil.  Species A and C have yet to be
encountered at the same locality.  The RAPD markers for
species C were found to be conserved over 4,620 km; from
Iguape, State of Spio Paulo, Brazil to Rio Socuavo, State of
Zulia, Venezuela.  RAPD-PCR was determined to be an effective
means for the identification of unknown specimens within this
species complex.

Anopheles (Nyssorhynchus) albitarsis -Anopheles (Nyssorhynchus) marajoara - Anopheles (Nyssorhynchus) deaneaorum - RAPD PCR - identification

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