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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 92, No. 5, 1997, pp. 613-617
Bioline Code: oc97115
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 92, No. 5, 1997, pp. 613-617

 en The Role of protein kinases in antigen-activation of peripheral blood mononuclear cells of Schistosoma mansoni infected individuals
Celia A Almeida & Alfredo M Goes

Abstract

T cell recognition of antigens displayed on the surface of antigen
presenting cell results in rapid activation of protein tyrosine kinases and
kinase C. This process leads to second messengers, such as inositol
phosphates and diacylgycerol, and phosphorylation of multiple proteins. The
role of different protein kinases in the activation of peripheral blood
mononuclear cells (PBMC) from Schistosoma mansoni infected individuals was
evaluated using genistein and H-7, specific inhibitors of protein tyrosine
kinase and kinase C, respectively. Our results showed that proliferation in
response to soluble egg antigen or adult worm antigen preparation of S.
mansoni was reduced when PBMC were cultured in presence of protein kinase
inhibitors. Using these inhibitors on in vitro granuloma reaction, we also
observed a marked reduction of granuloma index. Taken together, our results
suggest that S. mansoni antigen activation of PBMC involves protein kinases
activity.

Keywords
schistosomiasis - T cell activation - protein tyrosine kinase - protein kinase C

 
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