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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 92, No. 6, 1997, pp. 811-814
Bioline Code: oc97152
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 92, No. 6, 1997, pp. 811-814

 en Biological parameters and molecular markers of clone CL Brener - the reference organism of the Trypanosoma cruzi Genome Project
Bianca Zingales; Maria Elizabeth S Pereira; Katia A Almeida; Eufrosina S Umezawa; Nedia S Nehme; Riva P Oliveira; Andrea Macedo & Ricardo P Souto

Abstract

Clone CL Brener is the reference organism used in the Trypanosoma cruzi
Genome Project. Some biological parameters of CL Brener were determined:
(a) the doubling time of epimastigote forms cultured in liver
infusion-tryptose (LIT) medium at 28 C is 58+/-13 hr; (b) differentiation
of epimastigotes to metacyclic trypomastigotes is obtained by incubation in
LIT-20% Grace's medium; (c) trypomastigotes infect mammalian cultured cells
and perform the complete intracellular cycle at 33 and 37 C; (d) blood
forms are highly infective to mice; (e) blood forms are susceptible to
nifurtimox and benznidazole. The molecular typing of CL Brener has been
determined: (a) isoenzymatic profiles are characteristic of zymodeme ZB;
(b) PCR amplification of a 24Sa ribosomal RNA sequence indicates it belongs
to T. cruzi lineage 1; (c) schizodeme, randomly amplified polymorphic DNA
(RAPD) and DNA fingerprinting analyses were performed.

Keywords
CL Brener - Trypanosoma cruzi - Genome Project - biological characteristics - molecular typing

 
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