Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
Vol. 92, No. s2, 1997, pp. 197-200
Bioline Code: oc97186
Full paper language: English
Document type: Research Article
Document available free of charge
Memórias do Instituto Oswaldo Cruz, Vol. 92, No. s2, 1997, pp. 197-200
© Copyright 1997 Fundacao Oswaldo Cruz - Fiocruz
A Role For Lymphocytes And Cytokines On The Eosinophil Migration Induced By LPS|
Castro-Faria-Neto, Hugo C.; Penido, Carmen M.; Larangeira, Andrea P.; Silva, Adriana R. & Bozza, Patricia T.
In the present work we review the existing evidence for a LPS-induced
cytokine-mediated eosinophil accumulation in a model of acute inflammation.
Intrathoracic administration of LPS into rodents (mice, rats or guinea
pigs) induces a significant increase in the number of eosinophils recovered
from the pleural fluid 24 hr later. This phenomenon is preceded by a
neutrophil influx and accompanied by lymphocyte and monocyte accumulation.
The eosinophil accumulation induced by LPS is not affected by inhibitors of
cyclo or lipoxygenase nor by PAF antagonists but can be blocked by
dexamethasone or the protein synthesis inhibitor cycloheximide. Transfer of
cell-free pleural wash from LPS injected rats (LPS-PW) to naive recipient
animals induces a selective eosinophil accumulation within 24 hr. The
eosinophilotactic activity present on the LPS-PW has a molecular weight
ranging between 10 and 50 kDa and its effect is abolished by trypsin
digestion of the pleural wash indicating the proteic nature of this
activity. The production of the eosinophilotactic activity depends on the
interaction between macrophages and T-lymphocytes and its effect can not be
blocked by anti-IL-5 monoclonal antibodies. Accumulated evidence suggest
that the eosinophil accumulation induced by LPS is a consequence of a
eosinophilotactic cytokine produced through macrophage and T-cell
interactions in the site of a LPS-induced inflammatory reaction.
LPS - eosinophil - macrophages - T-cells - cytokine
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