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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 93, No. 2, 1998, pp. 247-254
Bioline Code: oc98047
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 93, No. 2, 1998, pp. 247-254

 en Molecular and antigenic characterization of the Leishmania (Viannia) panamensis kinetoplastid membrane protein-11
Jose R Ramirez; Christof Berberich; Andres Jaramillo; Carlos Alonso & Ivan D Velez

Abstract

The kinetoplastid membrane protein 11 (KMP-11) has been recently
described in Leishmania (Leishmania) donovani as a major component
of the promastigote membrane. Two oligonucleotide primers were synthesized
to PCR-amplify the entire coding region of New World Leishmania
species. The Leishmania (Viannia) panamensis amplification
product was cloned, sequenced and the putative amino acid sequence
determined. A remarkably high degree of sequence homology was observed with
the corresponding molecule of L. (L) donovani and L. (L)
infantum (97% and 96%, respectively). Southern blot analysis showed
that the KMP-11 locus is conformed by three copies of the gene. The L.
(V) panamensis ORF was subsequently cloned in a high expression vector
and the recombinant protein was induced and purified from Escherichia
coli cultures. Immunoblot analysis showed that 80%, 77% and 100% sera
from cutaneous, mucocutaneous and visceral leishmaniasis patients,
respectively, recognized the recombinant KMP-11 protein. In a similar
assay, 86% of asymptomatic Leishmania-infected individuals showed
IgG antibodies against the rKMP-11. We propose that KMP-11 could be used as
a serologic marker for infection and disease caused by Leishmania in
America.

Keywords
New World leishmaniasis - Leishmania (Viannia) panamensis - kinetoplastid membrane protein-11 - antigenicity

 
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