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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 93, No. 4, 1998, pp. 531-537
Bioline Code: oc98103
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 93, No. 4, 1998, pp. 531-537

 en Parasite genotypically related to a monoxenous trypanosomatid of dog's flea causing opportunistic infection in an HIV positive patient
Raquel S Pacheco; Mauro CA Marzochi; Marize Q Pires; Celia MM Brito; Maria de Fatima Madeira & Elizabeth GO Barbosa-Santos

Abstract

An HIV positive patient presenting a clinical picture of visceral
leishmaniasis co-infection was submitted to a bone marrow aspiration after
admission to hospital. Amastigotes forms were seen in the bone marrow
aspirate and the parasite grew in culture as promastigotes. Molecular
analyses showed that the flagellates isolated did not belong to the genera
Leishmania, Trypanosoma or Sauroleishmania. It was not
possible to establish infection in laboratory animals. In vitro
culture of mouse peritoneal macrophages revealed the invasion of the host
cells by the flagellates and their killing 48 hr after infection.
Opportunistic infection with an insect trypanosomatid was suspected.
Further hybridization analyses against a pannel of different monoxenous and
heteroxenous trypanosomatids showed kDNA cross-homology with Leptomonas
pulexsimulantis a trypanosomatid found in the dog's flea. {P}

Keywords
opportunistic infection - AIDS - monoxenous trypanosomatid - minicircle - hybridization

 
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