Gastroprotective effects of β3-adrenoceptor agonists on water immersion plus restraint stress-induced gastric ulcer in rats

OBJECTIVE: To evaluate the gastroprotective effects of β₃-adrenoceptor agonists CGP 12177A and SR 58611A, on water immersion plus restraint stress (WIRS)-induced gastric ulceration in rats. MATERIAL AND METHODS: Drugs were administered (5, 10 and 15 mg/kg, p.o.) 30 min prior to the ulcerogenic procedure. Ulcer index and the score for intensity of intraluminal bleeding were determined. Gastric wall mucus content (GWMC) and mast cell counts were determined in the glandular portion of the stomach. RESULTS: A dose-dependent reduction in the ulcer index was observed with both the drugs. A significant rise in the GWMC in the glandular tissue at 15 mg/kg dose was caused by the β₃-adrenoceptors agonists. In the glandular tissue the mast cell count was significantly decreased at 10 and 15 mg/kg dose with both drugs. CONCLUSION: The present study shows the gastroprotective effect of β₃-adrenoceptor agonists CGP 12177A and SR 58611A against WIRS-induced gastric ulceration in rats. The gastroprotective effect may be mediated by the enhancement of mucin activity and the decrease in mast cell degranulation.