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Indian Journal of Pharmacology
Medknow Publications on behalf of Indian Pharmacological Society
ISSN: 0253-7613
EISSN: 0253-7613
Vol. 38, No. 3, 2006, pp. 177-180
Bioline Code: ph06043
Full paper language: English
Document type: Research Article
Document available free of charge

Indian Journal of Pharmacology, Vol. 38, No. 3, 2006, pp. 177-180

 en Research Paper - Bactericidal activity of manganese (IV) complex of 2-methylamino-pyridine against Streptococcus pyogenes   check for this species in other resources and Staphylococcus aureus   check for this species in other resources
El-Masry S, El-Sayed IH, Lotfy Mahmoud, El-Shahat M, Ali SA, Nasif WA

Abstract

Objective: To investigate the bactericidal activity of manganese (IV) complex of 2-methylamino-pyridine against Streptococcus pyogenes   check for this species in other resources ( S. pyogenes ) and Staphylococcus aureus   check for this species in other resources ( S. aureus ).
Materials and Methods: The inhibitory effect of the complex was studied on the molecular level and by turbidity measurement. Treatment of bacteria was carried out using 5, 10, 25, 50 and 100 µmol of the complex per ml of culture media.
Results: The results showed that the growth of S. pyogenes rapidly decreased with increasing concentrations of the complex. In contrast, the complex caused no significant decrease in the growth rate of S. aureus . The molecular level studies showed that four protein bands, with apparent molecular weights of 19, 23, 30 and 54 Kda, respectively, increased in the protein pattern of the S. pyogenes extract after the complex treatment using silver stained polyacrylamide gels, under reducing condition . However, there was no detectable change in the protein pattern of the S. aureus extract after the complex treatment . No DNA damage was detected while using agarose gel electrophoresis and ethidium bromide staining in both types of bacteria.
Conclusion: Manganese (IV) complex of 2-methylamino-pyridine showed an apparent antibacterial inhibitory effect against S. pyogenes, but S. aureus was apparently resistant.

Keywords
Free radicals, gram-positive cocci, metal complex, SOD.

 
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