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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 8, No. 4, 2009, pp. 317-324
Bioline Code: pr09041
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 8, No. 4, 2009, pp. 317-324

 en Anti-Cancer Properties of Diethylether Extract of Wood from Sukun ( Artocarpus altilis check for this species in other resources ) in Human Breast Cancer (T47D) Cells
Arung, Enos Tangke; Wicaksono, Britanto Dani; Handoko, Yohana Ayupriyanti; Kusuma, Irawan Wijaya; Yulia, Dina & Sandra, Ferry

Abstract

Purpose: To evaluate the anti-cancer properties of the diethylether extract of Sukun ( Artocarpus altilis check for this species in other resources ) wood.
Methods: The extract was tested in human T47D breast cancer cells and examined for its effect on cell viability, nuclear morphology and sub-G1 formation. Cell viability was determined by microculture tetrazolium technique (MTT), nuclear morphology investigated using 4’-6-diamidino-2-phenylindole (DAPI) staining technique, and cell cycle progression monitored by sub-G1 apoptosis assay using flow cytometry.
Results: The results showed decreasing cell viability in a concentration-dependent manner. Altered cell morphology after treatment with the extract demonstrated that cells experienced apoptosis. Cell cycle analysis indicated that the number of cells in sub-G1 phase rose with increasing concentrations of the extract.
Conclusion: The data demonstrate that Sukun wood extract induced apoptosis and sub-G1 phase formation in breast cancer (T47D) cells, and therefore, has a potential as an anti-cancer agent.

Keywords
Artocarpus altili; Diethylether extract; T47D cells; Anti-cancer; Apoptosis; Sub-G1 formation

 
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