search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 8, No. 4, 2009, pp. 345-352
Bioline Code: pr09045
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 8, No. 4, 2009, pp. 345-352

 en Antiproliferative Effect of the Methanol Extract of Piper crocatum check for this species in other resources Ruiz & Pav Leaves on Human Breast (T47D) Cells In-vitro
Wicaksono, Britanto Dani; Handoko, Yohana Ayupriyanti; Arung, Enos Tangke; Kusuma, Irawan Wijaya; Yulia, Dina; Pancaputra, Aldrin Neilwan & Sandra, Ferry

Abstract

Purpose: To investigate the anti-cancer properties of the methanol extract of Piper crocatum check for this species in other resources Ruiz & Pav. leaves and its mode of action in human breast cancer (T47D) cells.
Methods: The anti-cancer property and mechanism of action of the extract were evaluated by its effect on cell viability, nuclear morphology, cell cycle progression and the expression of phosphorylated p44/p42 as a marker for cell proliferation.
Results: The results showed that there was a reduction of cell viability by the extract in a concentration-dependent manner and no alteration of nuclear morphology was observed. There were negligible changes in Sub-G1 phase formation after extract treatment. Expression of phosphorylated p44/42 was decreased by the extract only. Inclusion of the extract in the incubation medium decreased insulin-stimulated phosphorylation of p44/p42 indicating that the anti-proliferative effect of the extract was via p44/p42 pathway.
Conclusion: All together, the data indicate that P. crocatum methanol extract inhibits the growth of human breast cancer (T47D) cells via inhibition of p44/p42 phosphorylation.

Keywords
Piper crocatum leaf; methanol extract; antiproliferative; breast cancer; T47D cells

 
© © Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria.
Alternative site location: http://www.tjpr.org

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2022, Site last up-dated on 19-Jan-2022.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil