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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 8, No. 6, 2009, pp. 521-530
Bioline Code: pr09067
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 8, No. 6, 2009, pp. 521-530

 en Radioprotective and In-Vitro Cytotoxic Sapogenin from Euphorbia neriifolia check for this species in other resources (Euphorbiaceae) Leaf
Bigoniya, Papiya & Rana, Avtar Chand

Abstract

Purpose: Euphorbia neriifolia check for this species in other resources Linn. (Euphorbiaceae) plant is traditionally used in the treatment of abdominal troubles, bronchitis, tumours, leucoderma, piles, inflammation, and enlargement of spleen. The objective of this study was to evaluate the antioxidant and anticancer activities of a sapogenin isolate of this plant.
Methods: Euphol was isolated as a major constituent from the triterpenoidal sapogenin fraction of E. neriifolia leaf. Its in-vitro antioxidant activity was evaluated by reducing power assay, 1,1 – diphenyl –2- picryl hydrazyl (DPPH) assay, as well as hydroxyl radical and superoxide radical scavenging activities. Radioprotective activity was assessed against radiation-induced chromosomal aberrations and cytotoxicity on murine F1 B16 melanoma.
Results: The sapogenin exerted moderate antioxidant activity with highly significant (p < 0.001) reduction of gamma radiation-induced chromosomal aberrations (33.5 % compared to 71.5 % for radiation treatment alone at 4 Gy). It also exhibited cytotoxic activity on melanoma cell lines (IC50 = 173.78 μg/ml).
Conclusion: The sapogenin fraction showed antioxidant, radioprotective and cytotoxic activities. This study provides a scientific basis for the claimed traditional anticarcinogenic potentials of E. neriifolia.

Keywords
Euphorbia neriifolia; Euphol; Sapogenin; Antioxidant; Radioprotective; Melanoma; Chromosomal aberration

 
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