Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 8, No. 6, 2009, pp. 531-537
Bioline Code: pr09068
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 8, No. 6, 2009, pp. 531-537
© Copyright © Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria.
Development and Validation of a RP-HPLC Method for the Simultaneous Determination of Rifampicin and a Flavonoid Glycoside - A Novel Bioavailability Enhancer of Rifampicin|
Sachin, Bhusari S.; Bhat, Vandhna; Koul, Meenakshi; Sharma, Subhash C.; Tikoo, Manoj K.; Tikoo, Ashok K.; Satti, Naresh K.; Suri, Krishan A. & Johri, Rakesh K.
To develop and validate a sensitive HPLC method for the separation and simultaneous
estimation of two ingredients in a composition comprising of rifampicin and a flavonoid glycoside (an
enhancer of oral bioavailability of rifampicin).
Reverse phase (RP) chromatographic separation and estimation was achieved using a
Shimadzu HPLC system. RP-18 column was used at the following optimised conditions: mobile phase,
acetonitrile:phosphate buffer, 50 mM, pH 5.0 in a ratio of 60:40 v/v; oven temperature, 40 °C; flow rate,
0.8 ml min-1; detection wavelength, 340 nm; and total run time, 15 min.
The developed method was validated in terms of linearity, range, accuracy, precision, limit of
detection, limit of quantification, robustness and specificity. Good linearity was observed (r2 > 0.999)
over the study range of both ingredients. The precision values for rifampicin and the flavonoid glycoside
were in the range 1.08-2.77 and 1.14-2.98 %, respectively, while the limit of quantification was 0.10 and
0.05μg mL-1 respectively. The method was found to be robust and specific for both ingredients.
The developed method has a potential application in preclinical and clinical studies.
Flavonoid glycoside; RP-HPLC; Rifampicin; Stability studies; Validation
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