search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 10, No. 2, 2011, pp. 161-168
Bioline Code: pr11023
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 10, No. 2, 2011, pp. 161-168

 en Inhibition of Lipopolysaccharide-Induced iNOS, COX-2, and TNF-α Expression by Aqueous Extract of Orixa japonica check for this species in other resources in RAW 264.7 Cells via Suppression of NFκB Activity
Kang, Chang-Hee; Choi, Yung H; Choi, Il-Whan; Lee, Jae-Dong & Kim, Gi-Young

Abstract

Purpose: To investigate the anti-inflammatory effects of aqueous extract of Orixa japonica check for this species in other resources (AEOJ) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells.
Methods: The expression of mRNA and protein using RT-PCR and Western blot analysis was investigated. The level of nitric oxide (NO) production was analyzed using Griess reaction. Release of prostaglandin E2 (PGE2) and tumor necrosis factor-α (TNF-α) was determined using sandwich ELISA.
Results: AEOJ potently inhibited the production of nitric oxide (NO), prostaglandin E2 (PGE2 ), and tumor necrosis factor-α (TNF-α) in LPS-stimulated RAW 264.7 cells. Consistent with these findings, AEOJ was also found to significantly reduce LPS-induced expression of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), and TNF-α at the transcriptional level. Additionally, AEOJ attenuated LPSinduced NF-κB activity via the inhibition of IκB phosphorylation and degradation. It was also found that the NF-κB inhibitor N-acetyl cysteine (NAC) attenuated LPS-induced gene expression of iNOS, COX-2, and TNF-α. These results indicate that AEOJ attenuates LPS-induced inflammatory mediators such as NO, PGE2 , and TNF-α via suppression of NF-κB activity.
Conclusion: These results suggest that AEOJ has a potential activity to alleviate LPS-induced inflammation.

Keywords
Orixa japonica, Nitric oxide, Prostaglandin E2, Tumor necrosis factor-α, Nuclear factor-κB

 
© Copyright 2011 Tropical Journal of Pharmaceutical Research.
Alternative site location: http://www.tjpr.org

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2022, Site last up-dated on 10-Dec-2021.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil