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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 10, No. 2, 2011, pp. 211-218
Bioline Code: pr11029
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 10, No. 2, 2011, pp. 211-218

 en Phytochemical and Antibacterial Evaluations of the Stem Bark of Newbouldia laevis check for this species in other resources against Isolates from Infected Wounds and Eyes
Akerele, JO; Ayinde, BA & Ngiagah, J

Abstract

Purpose: To examine the phytochemical constituents and verify the ethnomedical claim of Newbouldia laevis check for this species in other resources (P.Beauv.) Seeman ex Bureau Bignoniaceae in treating septic wounds and eye problems.
Methods: Applying standard methods, the phytochemical constituents of the stem bark were examined while the antibacterial potentials of the methanol extract of the stem bark and its organic solvent fractions were tested on clinical bacterial isolates from infected wounds and eyes using the agar -well diffusion method. Ciprofloxacin and gentamicin were used as standard controls. The time-kill kinetics of the methanol stem bark extract and ciprofloxacin were determined using isolates of Staphyloccocus aureus check for this species in other resources .
Results: Phytochemical screening of the stem bark revealed the presence of flavonoids, tannins, saponins and alkaloids with no traces of cyanogenic glycosides. The 65 bacterial pathogens isolated included Proteus mirabilis check for this species in other resources (26.0 %) and Pseudomonas aeurginosa check for this species in other resources (17.4 %) from non-diabetic patients’ wounds; Staphylococcus aureus (32.0 %) and Escherichia coli check for this species in other resources (16.0%) from diabetic patients’ wounds; Staphylococcus aureus (35.3%) and Pseudomonas aeruginosa (35.3 %) from infected eyes. The chloroform fraction was observed to be more active on Gram-negative organisms while the aqueous fraction was more active on Gram-positive organisms. Time-kill kinetics of Staphylococcus aureus showed that the extract was bactericidal (99.9 % killing) at MIC and 2 x MIC after 24 and 3 hours, respectively.
Conclusion: The study has established that the stem bark of Newbouldia laevis has antibacterial activities against bacterial isolates from infected wounds and eyes as claimed in ethnomedicinal practice.

Keywords
Newbouldia laevis, Antibacterial, Phytochemical, Wound isolates, Eye isolates

 
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