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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996 EISSN: 1596-5996
Vol. 16, No. 2, 2017, pp. 371-378
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Bioline Code: pr17049
Full paper language: English
Document type: Research Article
Document available free of charge
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Tropical Journal of Pharmaceutical Research, Vol. 16, No. 2, 2017, pp. 371-378
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Anti-proliferative effect of Moringa oleifera Lam (Moringaceae) leaf extract on human colon cancer HCT116 cell line
Tragulpakseerojn, Jintana; Yamaguchi, Naoto; Pamonsinlapatham, Perayot; Wetwitayaklung, Penpun; Yoneyama, Tatsuro; Ishikawa, Naoki; Ishibashi, Masami & Apirakaramwong, Auayporn
Abstract
Purpose: To investigate the in vitro anti-proliferative effect and mechanism of action of Moringa oleifera
Lam. leaf extract on human colon carcinoma HCT116 cell line.
Methods: M. oleifera leaves were extracted with methanol. It was fractionated by Sephadex LH-20
column chromatography. Several fractions were identified by thin layer chromatography (TLC), proton
nuclear magnetic resonance (1H NMR) and mass spectrometry (MS). The growth inhibitory activity and
mechanism of action of the extracts in HCT116 colon cancer cells were investigated by 3-(4, 5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay and Western blotting.
Results: Successive fractions from M. oleifera leaf crude extracts by column chromatography were
combined into four pooled batches (MOL1 - MOL4) according to their absorbance at 260 nm and TLC
pattern. MOL2 and MOL3 contain astragalin and isoquercetin, respectively. The results from MTT assay
indicated that cell proliferation was significantly (p < 0.05) inhibited in a concentration-dependent
fashion, especially by MOL2, MOL3 and MOL4. MOL2 and MOL3 exhibited a stronger cell growth
inhibition than their major ingredients. The anti-proliferative activity of MOL2 - MOL4 in HCT116 colon
cancer cells was mediated by downregulation of ERK1/2 phosphorylation.
Conclusion: M. oleifera leaf extract has a strong anti-proliferative activity which is exerted by
decreasing ERK1/2 phosphorylation. Thus, the extract has a potential for use in cancer
chemoprevention.
Keywords
Moringa oleifera; Anti-proliferation; Colon cancer; AKT; ERK1/2 phosphorylation; Chemoprevention
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