Sub-Chronic Toxicity Study of Alfa-Cypermethrin in Rats

Alfa-cypermethrin (α-CP) a synthetic pyrethroid (type-II) insecticide, dissolved in dimethyl sulfoxide (DMSO-1 mL) was given consecutive daily orally for 60 days at 1/10 LD₅₀ (14.5 mg/kg). The animals were sacrificed on day 61^st. Biochemicals, cytochrome P450, b5 contents in liver along with antioxidant status, tissue residual concentration, and brain GABA level were studied. It increased the serum aminotransaminase (AST, ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) activities and blood glucose level significantly (p<0.05). It significantly (p<0.05) decreased and increases cytochrome P450 content and b5 content in liver respectively. It increased the malondialdehyde (MDA) level, and decreased the activities of catalase (CAT) and superoxide dismutase (SOD) and glycogen in liver significantly. The pyrethroid was considerable in amount in tissues. The GABA levels in cerebellum and in whole brain without cerebellums were decreased significantly (p<0.05). Therefore repeated daily oral dose toxicity of α-CP at 1/10 LD₅₀ altered biochemical parameters, decreased cytochrome P450 content, antioxidant status, and decreased brain GABA level.

Keywords
α-CP, Cytochrome P450, Antioxidants, Tissue residual concentration, Brain GABA level, Rat