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Tanzania Health Research Bulletin
Health User's Trust Fund (HRUTF)
ISSN: 0856-6496
Vol. 8, No. 3, 2006, pp. 149-154
Bioline Code: rb06028
Full paper language: English
Document type: Research Article
Document available free of charge

Tanzania Health Research Bulletin, Vol. 8, No. 3, 2006, pp. 149-154

 en A simple technique for the detection of anti-malarial drug formulations and their presence in human urine
Lugimban, L.; Malebo, H.M.; Segeja, M.D.; Akida, J.A.; Malle, L.N. & Lemnge, M.M.

Abstract

A simple, sensitive, specific assay technique for the detection and semi-quantification of chloroquine, amodiaquine, quinine, primaquine, sulfadoxine and pyrimethamine in formulations and in human urine by using thin layer chromatography (TLC) was developed and tested in the laboratory. The method involved developing test samples spotted on TLC chromatogram by diethylamine-toluene-isopropanol (1:4:5 v/v/v) as the eluting solvent. The solvent system diethylamine-toluene-isopropanol (1:4:5 v/v/v) enabled the elution and detection of all the tested antimalarial drugs in solution and those spiked in human urine. Detection limits for chloroquine, amodiaquine, quinine and primaquine were the lowest at 0.00025 mg/ml. Sulfadoxine exhibited a detection limit of 0.0005 mg/ml whereas that of pyrimethamine was 0.001 mg/ml. The results indicate the suitability of this technique in antimalarial drug quality and bioavailability studies. It is envisaged that this technique will adequately address the role of drug absorption and excretion in the chemotherapy of malaria as well as to detect types of antimalarial drugs commonly used in the community.

Keywords
TLC, bioassay, bioavailability, anti-malarial drugs, urine

 
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