search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


Iranian Journal of Reproductive Medicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
ISSN: 1680-6433
EISSN: 2008-2177
Vol. 3, No. 2, 2005, pp. 74-78
Bioline Code: rm05013
Full paper language: English
Document type: Research Article
Document available free of charge

Iranian Journal of Reproductive Medicine, Vol. 3, No. 2, 2005, pp. 74-78

 en The effects of cumulus cells on in vitro maturation of mouse germinal vesicle stage oocytes
Mahmoudi, Reza; Subhani, Aligholi; Pasbakhsh, Parichehr; Abolhasani, Farid; Amiri, Iraj; Salehnia, Mozhdeh & Etesam, Farideh

Abstract

Background: In vitro maturation (IVM) of oocytes is a promising technique to reduce the costs and avert the side-effects of gonadotropin stimulation for in vitro fertilization (IVF). The pregnancy rates from oocytes matured in vitroare much lower than those of in vivo stimulation cycles, indicating that optimization of IVM remains a challenge.
Objective: In this study, we investigated the effect of cumulus cells on maturation and fertilization rate of immature oocytes (Germinal vesicle).
Materials and Methods: Germinal vesicle (GV) oocytes were recovered from 6-8 weeks old Balb C female mice 48hr after injection of 10 IU pregnant mare serum gonadotropin (PMSG). Collected oocytes were divided into two groups. Group A: GV oocytes without cumulus (denuded oocyte). Group B: GV oocytes with cumulus cells (cumulus-oocyte complex). The oocytes in both groups were cultured inTCM-199 medium in a humidified atmosphere of 5% CO2 in air at 37ºC. The maturation, fertilization and developmental rates were recorded after 24hr.
Results: Maturation, fertilization and developmental rates in denuded oocytes (DO) were 65.1%, 68.02%, 78.63% respectively, and in cumulus-oocyte complex (COC) were 78.20%, 85.57% and 85.05%, respectively. The maturation, fertilization and developmental rates of COC were significantly higher than those of DO (p<0.05).
Conclusion: The results show that cumulus cells have beneficial effects on maturation, fertilization and cleavage rates of mice oocytes.

Keywords
Immature oocyte, In vitro maturation, Mouse, Cumulus cells

 
© Copyright 2005 - Iranian Journal of Reproductive Medicine
Alternative site location: http://www.ijrm.ir

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2017, Site last up-dated on 05-Dec-2017.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil