search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


Iranian Journal of Reproductive Medicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
ISSN: 1680-6433
EISSN: 2008-2177
Vol. 5, No. 2, 2007, pp. 45-50
Bioline Code: rm07011
Full paper language: English
Document type: Research Article
Document available free of charge

Iranian Journal of Reproductive Medicine, Vol. 5, No. 2, 2007, pp. 45-50

 en The effect of hyaluronic acid on motility, vitality and fertilization capability of mouse sperms after cryopreservation
Mitra Bakhtiari; Aligholi Sobhani; Mohammad Akbari; arichehr Pasbakhsh; Mehdi Abbasi; Azim Hedayatpoor; Fardin Amidi & Feridoon Sargolzaei

Abstract

Background: Various approaches have been used in the attempts to improve the quality of frozen–thawed mouse sperms. According to literatures, it seems that hyaluronic acid (HA) has an important role on the permeability and motility of sperms and their interaction with gametes.
Objective: For evaluation of HA supplementation on sperm characteristics and fertilization capability, we investigated the effect of different doses of HA on mouse sperm morphology, motility, vitality and fertilization capability after freezing and thawing.
Materials and Methods: The cauda epididymes was removed from 6 male mice with aseptic method. The sperm samples were frozen in 1.8 ml cryotubes with 18% raffinose and 3% skimmed milk containing cryo-protectant solution. HA at the concentration of 750, 1000 or 1250 μg/ml was supplemented to frozen-thawed sperms. Sperm motility wasmeasured with microscope, and fertilization rate was evaluated after routine IVF by counting the fertilized oocytes. For sperm morphology, papaniclau staining was used while; Eosin B was used for the assessment of sperm viability rate.
Results: HA supplementation (750 μg/ml) improved motility parameters (p < 0.05) and increased the fertility rate (p < 0.05). The effect of 1,000 μg/ml HA was also positive on the sperms. But 1,250 μg/ml HA had negative effect on above mentioned characteristic. On the other hand, none of these doses had any effect on sperm morphology.
Conclusion: The dose of 750 μg/ml of HA has the greatest effect on the motility, vitality and fertility rate of sperms after cryopreservation.

Keywords
Hyaluronic acid (HA), Cryopreservation, In vitro fertilization (IVF), Sperm parameters.

 
© Copyright 2007 - Iranian Journal of Reproductive Medicine
Alternative site location: http://www.ijrm.ir

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2017, Site last up-dated on 16-Oct-2017.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil