International Journal of Reproductive Medicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
Vol. 5, No. 4, 2007, pp. 177-181
Bioline Code: rm07034
Full paper language: English
Document type: Research Article
Document available free of charge
International Journal of Reproductive Medicine, Vol. 5, No. 4, 2007, pp. 177-181
© Copyright 2007 - Iranian Journal of Reproductive Medicine
Effects of melatonin on histopathological changes after experimental ovarian torsion-detorsion in cat|
Mostajeran, Fatemeh; Naderi, Maryam & Adibi, Shahriar
Background: During the detorsion of a torsioned ovary, oxidant agents are released and melatonin as an antioxidant can reduce ischemia. We studied the histopathological changes after using melatonin on experimental torsioned ovary in cat.
Objective: The aim of this experimental study was to investigate the effects of Melatonin on histopathological changes in torsion – detorsion injury in cat ovary.
Materials and Methods: An adnexal torsion – detorsion model was created by using 20 adult cats randomly divided equally in to 2 groups of Saline and Melatonin. Ischemia was induced by iathrogenic 360° clockwise torsion of the cat adnex for 3 hr. Reperfusion was achieved for 3 hr. Melatonin or saline were injected intra peritoneally (10mg/kg) 30 min before ovarian detorsion in both groups. After 3 hr of ovarian detorsion, ovarian tissue was removed and fixed in 10% formalin solution, embedded in paraffin and evaluated for ischemic indices.
Results: Histological examination showed a significant improvement in ovarian morphology in the melatonin treated cats. Edema and vasoconstriction in saline group were more severe than Melatonin group (p-value = 0.009). Hemorrhage and leukocyte infiltration were also more obvious in saline group (p-value 0.0018)
Conclusion: Our results demonstrated that Melatonin administration reduced ovarian histopathological damage due to oxidative injury associated with torsion.
Melatonin, Saline, Ischemia, Reperfusion, Cat.
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